Journal: International Journal of Molecular Sciences

Article Title: Specific Cerebrospinal Fluid SerpinA1 Isoform Pattern in Alzheimer’s Disease

doi: 10.3390/ijms23136922

Figure Lengend Snippet: Typical CIEF electropherograms of serpinA1 isoforms. Exemplary CIEF electropherograms of ( A ) control subjects (six distinct peaks in the pH range of 4.3–4.7), ( B ) patients with Alzheimer’s disease with dementia (AD-dem) (with a relative decrease in isoform 2 and an increase in peak 4) and ( C ) patients with Parkinson’s disease with dementia (PDD). The grey color indicates the area under each peak. Signal intensity is reported in chemiluminescence units (CUs). pI: isoelectric point; exposure time: 120 s.

Article Snippet: Analysis of CSF serpinA1 was performed in Ulm (Centre for biomedical research, University of Ulm, Ulm, Germany) using a NanoPro 1000 CIEF immunoassay platform, reagents (pH gradients, capillaries and fluorescent standards) and Compass software from ProteinSimple (Santa Clara, CA, USA), as previously described [ ].

Techniques: Control

Journal: The FEBS journal

Article Title: A novel 2D-based approach to the discovery of candidate substrates for the metalloendopeptidase meprin.

doi: 10.1111/j.1742-4658.2008.06592.x

Figure Lengend Snippet: Fig. 1. Simple 2D IEF ⁄ SDS ⁄ PAGE-based image analysis procedure. The procedure is based on qualitative differences among reference gels (level 1 match-sets) of each group of five gel replicates (three pooled biological gel replicates and two more technical gel repli- cates). Gel replicates of each group (activated meprin versus non-activated meprin) were cut virtually into four equally spaced quadrants for four independent image analyses. Reference gels of each group were then clustered into a new set for higher-level image analysis. The spot matching features of PDQUEST (version 7.3.1) allowed for detection of unique protein spots. The combined higher- level match-set is the final fusion of all annotated unique spots into one big 2D reference map.

Article Snippet: 2D IEF ⁄ SDS ⁄PAGE-based image analysis was performed using the program pdquest, version 7.3.1 (Bio-Rad Laboratories).

Techniques:

Journal: The FEBS journal

Article Title: A novel 2D-based approach to the discovery of candidate substrates for the metalloendopeptidase meprin.

doi: 10.1111/j.1742-4658.2008.06592.x

Figure Lengend Snippet: Fig. 2. Application of a simple 2D IEF ⁄ SDS ⁄ PAGE-based protease proteomic approach in substrate finding. A representative image analysis of the first quadrant is shown. Two hundred and fifty micrograms of conditioned medium protein from trypsin activated and non-activated MDCKa ⁄ b cells was separated by IEF in a 24 cm long IPG pH 3–10 NL strip. Vertical separation was according to mass in a 12.5% SDS gel. Optimized Ruthenium staining: for each condition (activated meprin versus non-activated meprin), three pooled biological gel replicates (from 18 dishes per pooled sample) and two more technical gel replicates (of one pooled sample) were produced for subsequent image analysis. Unique protein spots are labelled in level 1 and higher-level match-sets with SSP assigned by the image analysis software.

Article Snippet: 2D IEF ⁄ SDS ⁄PAGE-based image analysis was performed using the program pdquest, version 7.3.1 (Bio-Rad Laboratories).

Techniques: Stripping Membranes, SDS-Gel, Staining, Produced, Software